Using Poisson mixed-effects model to quantify transcript-level gene expression in RNA-Seq
نویسندگان
چکیده
منابع مشابه
Using Poisson mixed-effects model to quantify transcript-level gene expression in RNA-Seq
MOTIVATION RNA sequencing (RNA-Seq) is a powerful new technology for mapping and quantifying transcriptomes using ultra high-throughput next-generation sequencing technologies. Using deep sequencing, gene expression levels of all transcripts including novel ones can be quantified digitally. Although extremely promising, the massive amounts of data generated by RNA-Seq, substantial biases and un...
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متن کاملDifferential analyses for RNA-seq: transcript-level estimates improve gene-level inferences Supplementary Material
The sim2 data set consists of simulated sequencing reads from the human chromosome 1. The sequencing parameters as well as underlying TPM values for the 15,677 transcripts in one of the two simulated conditions were estimated using RSEM v1.2.21 [6] from the ERS326990 sample from the ArrayExpress data set with accession number E MTAB 1733. We simulated three biological replicates from each of tw...
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ژورنال
عنوان ژورنال: Bioinformatics
سال: 2011
ISSN: 1460-2059,1367-4803
DOI: 10.1093/bioinformatics/btr616